WebApr 13, 2024 · 从对照组或10μM CCCP处理组分离线粒体,与从稳定表达GFP-tagged KIF5B和mCherry-tagged DYNLL1的细胞系中分离的S-100 胞液片段共孵育,洗脱和分析线粒体募集的外源标记蛋白。 ... 发现过表达TSPAN4或TSPAN9的细胞、或动力蛋白敲除细胞,保护了CCCP处理后的呼吸潜力。 WebNov 28, 2024 · The mCherry coding sequence was inserted into TSPAN4 at the position targeted by the sgRNA. Thus, an mCherry-positive signal indicates that native TSPAN4 …
Pairing of integrins with ECM proteins determines
WebmCherry 613 .. 1320 = 708 bp 236 amino acids = 26.7 kDa. Product: monomeric derivative of DsRed fluorescent protein. mammalian codon-optimized: ori 4020 .. 4608 = 589 bp. high-copy-number ColE1/pMB1/pBR322/pUC origin of replication: ori 4020 .. 4608 = 589 bp. high-copy-number ... WebApplication Note. *Optimal dilutions/concentrations should be determined by the researcher. Application. Recommended Dilution. WB. 0.2-2.5 ug/ml. IHC-P. 2-10 ug/ml. Not tested in other applications. north lanarkshire council investment team
Addgene: CAG-mCherry
WebPlasmid CAG-mCherry from Dr. Jordan Green's lab contains the insert mCherry and is published in Regen. Eng. Transl. Med. 2024. This plasmid is available through Addgene. WebScale bars, 5 µm. b, NRK cells co-expressing Tspan4–GFP and Chmp4b–mCherry were photodamaged and time-lapse images were collected. a,b, Cells were imaged using … WebNov 18, 2024 · Extracellular vesicles (EVs) play an important role in the communication between tissues and cells. However, it is difficult to screen and trace EVs secreted by specific tissues in vivo, which affects the functional study of EVs in certain tissues under pathophysiological conditions. In this study, a Cre-dependent CD63flag-EGFP co … north lanarkshire council itrent login