Phosphinothricin-resistant
WebNov 6, 2006 · Selection for kanamycin-, phosphinothricin- and hygromycin B-resistance commonly takes 7-10 d and high seedling density and fungal contamination may result in failure to recover transformants. Results: A method for identifying transformed seedlings in as little as 3.25 d has been developed. WebThese results indicated that the extra intron at the 5 0 end of the phosphinothricin-resistance gene is required for its efficient expression in G. lucidum. To confirm these results, we also ...
Phosphinothricin-resistant
Did you know?
WebOct 4, 2009 · The transgenic plants were resistant to phosphinothricin up to 200 mg/l, … WebA bioassay was developed for identifying transgenic wheat plants based on De Block et …
WebOct 21, 2024 · The CRISPR vector for this study was used in a previously published pBAtC that is composed of the U6 promoter from Arabidopsis to express gRNA including CRISPR RNA scaffold, the 35S promoter originally from cauliflower mosaic virus (CaMV) to express Cas9 and the Basta TM (Phosphinothricin) resistance gene for the selection of the first ... WebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl- (methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am Main, Germany), which is a nonselective, postemergence herbicide used for weed control in orchards and vineyards 1.
WebPhosphinothricin. Resistance against PTT can be achieved by elevated levels of GS, which … WebNov 6, 2006 · The phosphinothricin-resistant seedlings were uniformly much greener than …
WebAug 21, 2024 · After 14 days of incubation at 30 °C, carboxin-resistant transformants were screened for phosphinothricin-resistance protein and GUS expression. The transformants expressing opbar were screened on CYM plates with 150 mg/L phosphinothricin.
WebJan 1, 2008 · The use of phosphinothricin resistance as selectable marker for genetic . transformation of grapevine. R. J ARDAK-J AMOUSSI 1), B. B OUAMAMA 1), A. M LIKI 1), A. G HORBEL 1) and G. M. R EUSTLE 2) earnings limit for filing taxesWebPlant resistance to phosphinothricin is conferred through the bar gene, and due to the fast degradation of phosphinothricin in soil and in water, it presents a low risk for environmental contamination. Product Specifications Phosphinothricin Glufosinate or Glufosinate ammonium Also known as Bayer’s Basta TM Formula: C 5 H 15 N 2 O 4 P csw jobs in kyearnings limit when on social securityWebWe have previously transformed rice (Oyrza sativa L.) with the maize ribosome-inactivating protein b-32 gene (Zmcrip3a) and the phosphinothricin resistance gene (bar). In the present study, Southern blot hybridization analysis of 56 primary fertile transformants resulted in distinct band patterns, indicating that all the transformants had been generated by … cs.wkefu.comWebJan 18, 2024 · One of the microbes that produce phosphinothricin, Streptomyces hygroscopicus, has an enzyme that inactivates phosphinothricin and glufosinate by acylating it. The gene (bar) encoding this... earnings management ethical issuesWebNov 25, 2014 · L-phosphinothricin (PPT) is a naturally occurring amino acid with herbicidal activity and it is active component of herbicide "Basta". PPT is a potent inhibitor of glutamine synthase and thus causes rapid increase in ammonia concentration in plants and this leads to death of plant cell. cswitvkWebJul 14, 2024 · In the current study, a chloroplast transformation vector harboring the phosphinothricin resistance gene (bar) as a selectable marker was established, and two short peptide-encoding genes (ant1 and ant2) driven by the endogenous psbA and rbcL promoters were cloned in this vector. The recombinant plasmid was transformed using a … cswkenoshaappointments csw-wi.com