Halo assay protocol
WebThe Autophagy LC3 HiBiT Reporter Assay System uses a luminescent LC3 reporter to quantitatively measure autophagic flux. Choose from one of two prepared reporter cell lines (HEK293 or U2OS) or stably transfect the reporter vector into the cell line of your choice. With one assay, you can verify results using three different methods: plate ... Webprotocol also has optional cell viability analysis for multiplexing with the NanoBRET™ assays. Figure 1. Overview of the five-step NanoBRET™ Ternary Complex Assay. First, HaloTag®-VHL or HaloTag®-CRBN acceptor fusion and NanoLuc® or HiBiT donor fusion are expressed in cells. Second, the cells
Halo assay protocol
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WebJan 1, 2009 · The fast-halo assay (FHA) is a very recent method to detect DNA-strand breakage induced either by various genotoxic agents or secondary to apoptotic DNA cleavage, and to study the repair of primary DNA breaks at the single-cell level. ... Cite this protocol. Sestili, P. (2009). The Fast-Halo Assay for the Assessment of DNA Damage … WebNational Center for Biotechnology Information
WebThe pull-down assay is an in vitro method used to determine a physical interaction between two or more proteins. Pull-down assays are useful for both confirming the existence of a protein–protein interaction predicted … WebHT-HS10. Halosperm ® is an in vitro diagnostic kit that allows the measurement of DNA fragmentation in a fast, easy, and reproducible manner, without the need for complex laboratory equipment. Halosperm ® allows for an improved evaluation of sperm quality, complementing the information offered by the conventional sperm analysis. Conventional …
WebMay 24, 2024 · We here describe the implementation of this novel method, which we refer to as halo assay, and its validation with plasmin, urokinase and tissue plasminogen … WebDNA in the enrichment protocol can adversely affect your results. Use a fluorometry-based DNA quantitation method, such as PicoGreen stain ... Quant-iT dsDNA BR Assay Kit, for …
WebHaloTag® Technology: A Powerful Tool for Protein Labeling and Analysis Start by adding the HaloTag® protein fusion tag to your protein, pair the fusion protein with one of many …
WebApr 14, 2024 · The functional expression of the purinergic halo in human pDCs described in this work opens new areas to investigate its participation in the regulatory pDC mechanisms in health and disease. ... Supernatants were thawed at 4 °C, and the IFN-α assay (Invitrogen ThermoFisher, detection limit 3.2 pg/mL) was done according to the … rthfgbnWebSep 5, 2006 · The behaviour of single-stranded DNA fragments under the conditions of the modified assay, called fast halo assay (FHA), is essentially the same as in AHA. The modifications consist in the simplification of the lysis, denaturation and staining procedures, and allow, as compared with AHA, the preparation of samples within 15 min, with a two ... rtherm oneWebHaloPlex HS Custom Kits offer amplicon capture-based target enrichment with ultra-high sensitivity. By tagging every molecule with an unique molecular barcode, and capturing DNA from both strands, HaloPlex HS enables confident detection of rare variants with significantly improved base calling accuracy. These kits utilize a fast workflow of as little as under 6 … rtherjWebHaloTag® Technology: A Powerful Tool for Protein Labeling and Analysis. Start by adding the HaloTag® protein fusion tag to your protein, pair the fusion protein with one of many interchangeable ligands that forms a highly specific covalent bond with HaloTag, and you open a gateway to investigate protein function. Once your protein of interest ... rthevWebThe HaloCHIP™ System is a novel system that covalently captures crosslinked protein:DNA complexes from cells without the need for antibodies, providing a robust and efficient … rther1947WebDNA in the enrichment protocol can adversely affect your results. Use a fluorometry-based DNA quantitation method, such as PicoGreen stain ... Quant-iT dsDNA BR Assay Kit, for use with the Qubit fluorometer 100 assays, 2-1000 ng 500 assays, 2-1000 ng Thermo Fisher Scientific p/n Q32850 rtherWebSep 13, 2024 · 2. Protocol Summary Actin polymerization assay Prepare all controls and samples according to the protocol. Mix well and incubate for 15 minutes. Add 10 µL of Supplemented Buffer G and 10 µL of Supplemented Buffer P to the background and positive control respectively. Measure Fluorescence Ex/Em: 365/410 nm Actin Depolymerization … rthfdg