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WebNonspecific binding of monoclonal antibodies (mAbs) to Fc-receptors on leukocytes is an important cause of background fluorescence in flow cytometry, and failing to … WebSep 3, 2009 · APC-Cy7 also binds nonspecifically to monocytes (Beavis AJ, Pennline KJ. Allo-7: a new fluorescent tandem dye for use in flow cytometry. Cytometry. 1996; … red ball jets shoes commercial

Commonly Used Fluorochromes and Their Properties

WebPolymer Dyes. The second type of dye-mediated binding also relates to a specific group of (mostly tandem) dyes but is independent of the stained cells, i.e. it is due to the dye-dye interactions between different polymer … WebNonspecific binding of conjugated antibodies represents a critical step which could significantly influence the results of immunostaining or flow cytometry. In this respect, various staining procedures and distinct cell types can alter the results obtained with different fluorochromes. WebIsotype controls are used to determine the background caused by nonspecific antibody binding. An isotype control uses an antibody of the same isotype as the primary antibody but is specific for an antigen … red ball jumping bounce