WebCarboxymethylproline synthase was shown to condense l-glutamate semialdehyde with malonyl-coenzyme A to produce (2S,5S)-carboxymethylproline, while incubation of d-glutamate semialdehyde results only in uncoupled turnover of malonyl-CoA. WebNov 7, 2024 · Carbapenems are biologically mainly active in the infections like urinary tract infections, bloodstream infections, tuberculosis, intra-abdominal infections, and pathogens like anaerobes, gram-positive and gram-negative bacteria. Keywords: Carbapenem, antibiotics, structure, chemistry, resistance, mechanism of action. « Previous Next »
Carboxymethylproline Synthase (CarB), an Unusual Carbon-Carbon …
WebJan 1, 2005 · The simplest carbapenem antibiotic, (5R)-carbapen-2-em-3-carboxylic acid, is biosynthesized from primary metabolites in Pectobacterium carotorova by the action of three enzymes,... WebThe biocatalytic versatility of wildtype and engineered carboxymethylproline synthases (CMPSs) is demonstrated by the … businessoulu työllisyyspalvelut
Coupling carboxymethylproline synthases with alkylmalonyl-CoA …
WebCarB catalyzes formation of (2 S ,5 S )-carboxymethylproline [ (2 S ,5 S )- t -CMP] from malonyl-CoA and l -glutamate semialdehyde. In addition to using a cosubstrate, CarB catalyzes C-C and C-N bond formation processes as well as an acyl-coenzyme A … WebThe utility of wild-type and variant carboxymethylproline synthases for biocatalysis was demonstrated by preparing functionalised 5-, 6- and 7-membered N-heterocycles from … WebMay 1, 2011 · We describe protein engineering studies on 5-carboxymethylproline synthases, members of the crotonase superfamily, aimed at enabling stereoselective C-C bond formation leading to N-heterocycles... businessoulu palkkatukipaikat